The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs

Here are the The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs journals presenting the latest research across various disciplines. From social sciences to technology, each article is expected to provide valuable insights to our readers.

Proteomics dedicated to bioflinology, what is proteomics in biology, expert review of proteomics, what is proteomics quizlet, plasma proteomic profiles predict, proteomics journal impact factor, expert review of proteomics impact factor, proteomic profiling reveals.

The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs

To reveal the sources of obesity and type 2 diabetes (T2D) in humans, animal models, mainly rodents, have been used. Here, we propose a pig model of T2D. Weaned piglets were fed high fat/high sugar diet suppling 150% of metabolizable energy. Measurements of weight gain, blood morphology, glucose plasma levels, cholesterol, and triglycerides, as well as glucose tolerance (oral glucose tolerance test, OGTT) were employed to observe T2D development. The histology and mass spectrometry analyses were made post mortem. Within 6 months, the high fat-high sugar (HFHS) fed pigs showed gradual and significant increase in plasma triglycerides and glucose levels in comparison to the controls. Using OGTT test, we found stable glucose intolerance in 10 out of 14 HFHS pigs. Mass spectrometry analysis indicated significant changes in 330 proteins in the intestine, liver, and pancreas of the HFHS pigs. These pigs showed also an increase in DNA base modifications and elevated level of the ALK BH proteins in the tissues. Six diabetic HFHS pigs underwent Scopinaro bariatric surgery restoring glycaemia one month after surgery. In conclusion, a high energy diet applied to piglets resulted in the development of hyperlipidaemia, hyperglycaemia, and type 2 diabetes being reversed by a bariatric procedure, excluding the proteomic profile utill one month after the surgery. © The Author(s) 2024.

Authors : Ferenc K.; Marcinkowski M.; Olszewski J.; Kowalczyk P.; Pilžys T.; Garbicz D.; Dib N.; Świderska B.; Matyba P.; Gajewski Z.; Grzesiuk E.; Zabielski R.

Source : Nature Research